Monday, January 30, 2017

pGLO Lab



Plate
Number of Colonies
Color of colonies under room light
Color of colonies under   UV light
- pGLO LB
About 19
About 20
none
- pGLO LB/amp
none
none
none
+ pGLO LB/amp
About 20
About 19
none
+ pGLO LB/amp/ara
At least 55
At least 60
At least 45
The new traits include the bacteria being able to glow and another trait was them growing into colonies. I would estimate about 65 colonies, for the ones that lit up with UV light, there were about 50 colonies. There were more colonies when the UV was not on. Arabinose’s role was to allow the bacteria to glow under the UV light. The glow was a neon green. GFP has been used to make cancer cells glow green in order to see the amount and where the cancer cells are. Also to see the cancer cells spread in a translucent skin. In science, it is also used in biosensors, which can sense by sensing the pH or ion level in a thing, and it displaying the results in what colour the protein glows. Scientists have also recently used it to colour animals and other organisms. Another application of genetic engineering would be to test specific genes and cells to see their direct outcome and effects in different organisms.



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