pGLO Lab

Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB About 19 About 20 none - pGLO LB/amp none none none + pGLO LB/amp About 20 About 19 none + pGLO LB/amp/ara At least 55 At least 60 At least 45

The new traits include the bacteria being able to glow and another trait was them growing into colonies. I would estimate about 65 colonies, for the ones that lit up with UV light, there were about 50 colonies. There were more colonies when the UV was not on. Arabinose’s role was to allow the bacteria to glow under the UV light. The glow was a neon green. GFP has been used to make cancer cells glow green in order to see the amount and where the cancer cells are. Also to see the cancer cells spread in a translucent skin. In science, it is also used in biosensors, which can sense by sensing the pH or ion level in a thing, and it displaying the results in what colour the protein glows. Scientists have also recently used it to colour animals and other organisms. Another application of genetic engineering would be to test specific genes and cells to see their direct outcome and effects in different organisms.

Candy Electrophoresis Lab

1) We could not analyze the data, for as we went to an assembly, the teacher directed us to keeping the current on, which then resulted in the DNA moving off the gel. By analyzing some other groups gel, which also ran for too long, put some remained, we observed that the red became pink. Because this is not ours, we can not be sure of where the dyes were placed in the gel originally. We could also observe that all the dyes and the standard dyes moved at the same speed, meaning they were at the same place.

2) The Fast Green FCF will go as fast the dye found in Skittles, for the Skittles went the farthest and was green and the name of that dye is Fast Green FCF. We also can think that Citrus Red 2 will go as fast as the Mike n Ike, for those are the same colour.

3) They might put dyes into dog food, so that the dog owner might be more attracted into buying a dog food that is not just brown and might show that it has vegetables or other ingredients.

5) The size of the DNA and the amount of time will affect how far it travels during the experiment. The longer DNA, the less it will go far. Time will let them move the farthest they can.

6) The electrical current helps it move through the gel. They are attracted to the other side so they move.

7) Electricity is the component that causes the DNA to make groups by their size. Because they are repelled the negative charge, the DNA starts its way to the other side. But because the shorter DNA moves quickest, it goes farther, so each length goes a certain distance which can be compared to know DNA.

8) The DNA with the weight of 600 daltons will move faster, for it is smaller meaning it will be able to move the farthest. 5,000 daltons will move the least far, for because of its weight, it will travel very slowly towards the positive charge. From farthest to closest, 600 daltons, 1,000 dlatons, 2,000 daltons, and then 5,000 daltons.

Here is the experiment of the other group that worked.

Second Semester Goals

  My first goal, specifically for Biology class, will be to study for tests earlier and studying more proactively. I will accomplish this goal by starting to study well before the day of the test. I will also study more proactively by studying the main concepts, but go more in depth in the topics, I may have not understood as well as the others. I will also evenly space the amount of time studying throughout the days, such as 30 minutes a day. I will also try to get a better sleep prior to the day of the test. By doing this, not only will I better my studying skills, but I will also do better on test, but most importantly I will better remember the material being taught, even after the unit has ended.

   My second goal, which is broader, will be to not procrastinate on work and project given at school. I will achieve this goal by starting to accomplish the work when it is first given. I will lay out the work over the days, if I an extended period of time, such as chunks of 30-45 minutes of working on it. If it is just work due in a couple of days later, I will try to start it in class or at school, so once I come home, I will have less work to do, and ultimately finish the work in that day. By accomplishing this goal, I will stop being a procrastinator and will have good habits to when I go to college and even after that.